ABSTRACT
Object A study on the ingenol-type diterpene esters of Euphorbia kansui T. N. Liou ex T. P. Wang. Methods Their structures were elucidated by spectral analysis, especially 2DNMR ( 1H- 1HCOSY, HSQC, HMBC and NOESY). Results Six ingenol-type diterpene esters were isolated successfully, two of them were identified as 3-O-(2′E, 4′Z-decadienoyl)-20-O-acetylingenol (Ⅰ) and kansuiphorin C (Ⅱ). Conclusion NMR data of compound Ⅰ and 13 CNMR data of compound Ⅱ were first reported. On the basis of variety of 2DNMR experiments, NMR data of compound Ⅰ and Ⅱ were well assigned.
ABSTRACT
Object To study the chemical constituents of Pueraria omeiensis Tang et Wang. Methods Isolation and purification were repeatedly carried out on silica gel column chromatography, Sephadex LH-20, and preparative HPLC. The structures were identified and elucidated by physicochemical properties and spectral analysis. Results One compound was elucidated as sophoracoumestan A (Ⅰ) from the acetic ether section, and 15 compounds were obtained from the n-butanol section. They were ononin, (Ⅱ), daidzein 7,4′-diglucoside (Ⅲ), daidzein 7,4′-glucoside (Ⅳ), 3′-methoxy puerarin, PG-3 (Ⅴ), 3′- hydroxypuerarin, PG-1 (Ⅵ), mirificin (Ⅶ), 8-methyl retusin 7-O-glucoside (Ⅷ), daidzein (Ⅸ), daidzin (Ⅹ), genistin (Ⅺ), puerarin ( ⅩⅡ), octacosanoic ( ⅩⅢ), allantoin ( ⅩⅣ), glycerol 1-monopentacosanoate ( ⅩⅤ), daucosterol, ⅩⅥ), respectively. Conclusion Besides compounds Ⅸ- ⅩⅡ, ⅩⅥfrom the acetic ether section, the others are first obtained in P. omeiensis, and compounds Ⅰ, Ⅳ, Ⅷ are found in the plants of Puerarea DC. for the first time.
ABSTRACT
AIM To study an analytical method of fingerprint for Adina pilulifera (Lam.)Franch. ex Drake in order to ensure the quality of this medicinal material. METHODS RP-HPLC was used to detect 6 batches of this medicinal material collected in main production areas. The experiment was carried out by using Agilent ZORBAX Extend C_ 18 column (4.6 mm?250 mm), methanol:water (23∶ 77) and 258 nm. RESULTS This RP-HPLC method was accurate, stable and reproducible, and the main peaks in the spectrum were all separated perfectly. CONCLUSION The developed method is simple, accurate and operable, and can be used to control the quality of Adina pilulifera (Lam.)Franch.ex Drake efficiently and feasibly.